人源批量可大明胶新方法生产

2025-05-04 20:10:15分类：百科阅读(418)

人源批量可大明胶新方法生产

生物探索推荐英文论文摘要：

人源批量可大明胶新方法生产

New Strategy for Expression of Recombinant Hydroxylated Human-Derived Gelatin in Pichia pastoris KM71

人源批量可大明胶新方法生产

Abstract

Gelatin is 新方a well-known biopolymer, and it has a long history of use mainly as a gelling agent in the food industry. This paper reports a new method for producing recombinant hydroxylated human-derived gelatin in Pichia pastoris KM71. Three independent expression cassettes encoding for specific length of gelatin, prolyl 4-hydroxylase (P4H, EC 1.14.11.2), α-subunit (αP4H), and protein-disulfide isomerase (PDI) were individually cloned in one expression vector, pPIC9K. The modified gelatin gene and two subunit genes of P4H were under the control of two different inducible promoters, namely, alcohol oxidase 1 promoter (PAOX1) and formaldehyde dehydrogenase 1 promoter (PFLD1), respectively. The results of sodium dodecylsulfate-polyacrylamide gel electrophoresis show that a recombinant gelatin was successfully expressed in P. pastoris KM71 by methanol induction. Liquid chromatography coupled with tandem mass spectrometry analysis indicates that the expressed gelatin was hydroxylated with approximately 66.7% of proline residues in the Y positions of Gly-X-Y triplets. The results of nuclear magnetic resonance spectroscopy of recombinant gelatin test show that the 1H and 13C spectra have many corresponding characteristic displacement peaks, and amino acids composition analysis shows that it contains hydroxyproline and its UV absorption is consistent with the characteristics of gelatin.

Keywords: recombinant gelatin; Pichia pastoris KM71; coexpression; prolyl 4-hydroxylase; hydroxylation

而且可能引起一些人的大批免疫系统的应答。各批次的量生质量不一，这组科学家开发并证明了一种方法，产人基于动物的源明明胶大多数是用牛和猪的骨骼和皮肤制成的，

为了克服这些困难，新方

Jinchun Chen及其同事解释说，大批该研究发表在了美国化学会的量生《农业与食品化学期刊》（Journal of Agriculture and Food Chemistry）上。它可能带来诸如“疯牛病”等传染病的产人风险，这组科研人员还在测试人-酵母明胶从而弄清楚它与其他明胶相比粘稠度和其他属性如何。源明后者可以制造明胶且具有可控的新方特征。例如，大批这可以替代每年生产的量生30万吨动物明胶的一部分。

摘要：科学家报告说，产人这可以替代为明胶类型的源明甜点、他们开发了一种大批量生产基于人的明胶的新方法，这为制造商带来了困难。可能用于药物胶囊和其他医学应用。Chen和他的同事提出，